FLInt: single shot safe harbor transgene integration via <i>F</i>luorescent <i>L</i>andmark <i>Int</i>erference

Abstract

AbstractThe stable incorporation of transgenes and recombinant DNA material into the host genome is a bottleneck in many bioengineering applications. Due to the low efficiency, identifying the transgenic animals is often a needle in the haystack. Thus, optimal conditions require efficient screening procedures, but also known and safe landing sites that do not interfere with host expression, low input material and strong expression from the new locus. Here, we leverage an existing library of ≈Caenorhabditis elegans