Communications Biology (Nov 2022)
Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
- Anna Billeschou Bomholt,
- Christian Dall Johansen,
- Jens Bager Christensen,
- Sasha Alexandra Sampson Kjeldsen,
- Katrine Douglas Galsgaard,
- Marie Winther-Sørensen,
- Reza Serizawa,
- Mads Hornum,
- Esteban Porrini,
- Jens Pedersen,
- Cathrine Ørskov,
- Lise Lotte Gluud,
- Charlotte Mehlin Sørensen,
- Jens Juul Holst,
- Reidar Albrechtsen,
- Nicolai Jacob Wewer Albrechtsen
Affiliations
- Anna Billeschou Bomholt
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Christian Dall Johansen
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Jens Bager Christensen
- Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
- Sasha Alexandra Sampson Kjeldsen
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Katrine Douglas Galsgaard
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Marie Winther-Sørensen
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Reza Serizawa
- Department of Pathology, Copenhagen University Hospital
- Mads Hornum
- Department of Nephrology, Centre for Cancer and Organ Diseases, Rigshospitalet, University of Copenhagen
- Esteban Porrini
- Instituto de Tecnologías Biomédicas, University of La Laguna, Research Unit, Hospital Universitario de Canarias
- Jens Pedersen
- Department of Clinical Medicine, Faculty of Health Sciences, University of Copenhagen
- Cathrine Ørskov
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Lise Lotte Gluud
- Department of Clinical Medicine, Faculty of Health Sciences, University of Copenhagen
- Charlotte Mehlin Sørensen
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Jens Juul Holst
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- Reidar Albrechtsen
- Biotech Research & Innovation Centre, University of Copenhagen
- Nicolai Jacob Wewer Albrechtsen
- Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen
- DOI
- https://doi.org/10.1038/s42003-022-04242-7
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 13
Abstract
An assessment of all commercially available antibodies targeting the G-protein-coupled receptor (GPCR), glucagon receptor (GCGR) is used to highlight the most reliable antibody in immunostaining, including an antibody-independent verification.