Loss of ZBTB20 impairs circadian output and leads to unimodal behavioral rhythms

Zhipeng Qu; Hai Zhang; Moli Huang; Guangsen Shi; Zhiwei Liu; Pancheng Xie; Hui Li; Wei Wang; Guoqiang Xu; Yang Zhang; Ling Yang; Guocun Huang; Joseph S Takahashi; Weiping J Zhang; Ying Xu

doi:10.7554/eLife.17171

eLife (Sep 2016)

Loss of ZBTB20 impairs circadian output and leads to unimodal behavioral rhythms

Zhipeng Qu,
Hai Zhang,
Moli Huang,
Guangsen Shi,
Zhiwei Liu,
Pancheng Xie,
Hui Li,
Wei Wang,
Guoqiang Xu,
Yang Zhang,
Ling Yang,
Guocun Huang,
Joseph S Takahashi,
Weiping J Zhang,
Ying Xu

Affiliations

Zhipeng Qu: MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China
Hai Zhang: Department of Pathophysiology, Second Military Medical University, Shanghai, China
Moli Huang: Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China
Guangsen Shi: MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China
Zhiwei Liu: Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China
Pancheng Xie: ORCiD; MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China
Hui Li: Department of Pathophysiology, Second Military Medical University, Shanghai, China
Wei Wang: Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China
Guoqiang Xu: College of Pharmaceutical Sciences, Soochow University, Suzhou, China
Yang Zhang: Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China
Ling Yang: Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China
Guocun Huang: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, United States
Joseph S Takahashi: ORCiD; Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, United States
Weiping J Zhang: ORCiD; Department of Pathophysiology, Second Military Medical University, Shanghai, China
Ying Xu: ORCiD; MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing, China; Cambridge-Suda Genomic Research Center, Soochow University, Suzhou, China

DOI: https://doi.org/10.7554/eLife.17171
Journal volume & issue: Vol. 5

Abstract

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Many animals display morning and evening bimodal activities in the day/night cycle. However, little is known regarding the potential components involved in the regulation of bimodal behavioral rhythms in mammals. Here, we identified that the zinc finger protein gene Zbtb20 plays a crucial role in the regulation of bimodal activities in mice. Depletion of Zbtb20 in nerve system resulted in the loss of early evening activity, but the increase of morning activity. We found that Zbtb20-deficient mice exhibited a pronounced decrease in the expression of Prokr2 and resembled phenotypes of Prok2 and Prokr2-knockout mice. Injection of adeno-associated virus-double-floxed Prokr2 in suprachiasmatic nucleus could partly restore evening activity in Nestin-Cre; Zbtb20fl/fl (NS-ZB20KO) mice. Furthermore, loss of Zbtb20 in Foxg1 loci, but intact in the suprachiasmatic nucleus, was not responsible for the unimodal activity of NS-ZB20KO mice. Our study provides evidence that ZBTB20-mediated PROKR2 signaling is critical for the evening behavioral rhythms.

Published in eLife

ISSN: 2050-084X (Online)
Publisher: eLife Sciences Publications Ltd
Country of publisher: United Kingdom
LCC subjects: Medicine; Science: Biology (General)
Website: https://elifesciences.org

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