Lipid profiling analyses from mouse models and human infants

Laurentya Olga; Ivana Bobeldijk-Pastorova; Richard C. Bas; Florine Seidel; Stuart G. Snowden; Samuel Furse; Ken K. Ong; Robert Kleemann; Albert Koulman

STAR Protocols (Dec 2022)

Lipid profiling analyses from mouse models and human infants

Laurentya Olga,
Ivana Bobeldijk-Pastorova,
Richard C. Bas,
Florine Seidel,
Stuart G. Snowden,
Samuel Furse,
Ken K. Ong,
Robert Kleemann,
Albert Koulman

Affiliations

Laurentya Olga: Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK
Ivana Bobeldijk-Pastorova: Department of Metabolic Health Research, The Netherlands Organization for Applied Scientific Research (TNO), 2333 CK Leiden, the Netherlands
Richard C. Bas: DUCARES B.V. | trading as TRISKELION, Reactorweg 47-A, 3542 AD Utrecht, the Netherlands
Florine Seidel: DUCARES B.V. | trading as TRISKELION, Reactorweg 47-A, 3542 AD Utrecht, the Netherlands
Stuart G. Snowden: Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK
Samuel Furse: Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK
Ken K. Ong: Department of Paediatrics, University of Cambridge, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK
Robert Kleemann: Department of Vascular Surgery, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands
Albert Koulman: Core Metabolomics and Lipidomics Laboratory, Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0QQ, UK; MRC Epidemiology Unit, Wellcome Trust-MRC Institute of Metabolic Science, NIHR Cambridge Comprehensive Biomedical Research Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge CB2 0SL, UK; Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK; Corresponding author

Journal volume & issue: Vol. 3, no. 4
p. 101679

Abstract

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Summary: This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort.For complete details on the use and execution of this protocol, please refer to Olga et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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