Plasma oxidant/antioxidant status in period from the day of ovulation until embrional fixation day of pregnancy in mares

Abstract

This study was conducted to investigate plasma estradiol 17f3 and progesteron profiles, oxidant/ antioxidant status and the relationship among these parameters during the period Irom the day of ovulation until emb-rional lixation day in mares. Blood samples were collected on the day of ovulation (d=0) and on days 5, 10. and 16 ol pregnancy in 12 purebred mares. Plasma was analyzed for oxidant (ma I ond i aldehyde, index ol lipid peroxidation) and antioxidants (Vitamin E, (3-carotene, glutathione, catalase, glutathione peroxidase) values. Estradiol 17(3 level decreased after ovulation while, progesteron level gradually increased (p<0.05). Plasma malondialdehyde level on days 5 and 16 of pregnancy significantly were higher than d 0 (p<0.05). Plasma antioxidant parameters except for vitamin E gradually increased and this increase on 16th day was significantly higher than on day of ovulation day. Moreover, a negative correlation was observed (r=-0.50) between the plasma estradiol 17R and malondialdehyde concentrations. In conclusion, plasma malondialdehyde level generally increased, however the concentrations of 0-carotene, glutathione and activities of glutathione peroxidase and catalase parallelly increased during the period from the day ol ovulation until embrional fixation day in mares.

Keywords

• antioxidant
• oxidant
• early pregnancy
• mare