Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum

Baoling Li; Haizhen Wu; Na Li; Jiayuan Su; Ruilian Jia; Jianlin Jiang; Yaoyu Feng; Yaoyu Feng; Lihua Xiao

doi:10.3389/fmicb.2017.01647

Frontiers in Microbiology (Aug 2017)

Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum

Baoling Li,
Haizhen Wu,
Na Li,
Jiayuan Su,
Ruilian Jia,
Jianlin Jiang,
Yaoyu Feng,
Yaoyu Feng,
Lihua Xiao

Affiliations

Baoling Li: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and TechnologyShanghai, China
Haizhen Wu: School of Biotechnology, East China University of Science and TechnologyShanghai, China
Na Li: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China
Jiayuan Su: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and TechnologyShanghai, China
Ruilian Jia: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and TechnologyShanghai, China
Jianlin Jiang: Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, AtlantaGA, United States
Yaoyu Feng: State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and TechnologyShanghai, China
Yaoyu Feng: College of Veterinary Medicine, South China Agricultural UniversityGuangzhou, China
Lihua Xiao: Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, AtlantaGA, United States

DOI: https://doi.org/10.3389/fmicb.2017.01647
Journal volume & issue: Vol. 8

Abstract

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Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted Cryptosporidium species have different numbers of subtelomeric genes encoding the Cryptosporidium-specific MEDLE family of secreted proteins, which could contribute to differences in host specificity. In this study, a Cryptosporidium parvum-specific member of the protein family MEDLE-2 encoded by cgd5_4590 was cloned and expressed in Escherichia coli. Immunofluorescent staining with antibodies generated from the recombinant protein showed the expression of the protein in sporozoites and development stages. In vitro neutralization assay with the antibodies partially blocked the invasion of sporozoites. These results support the potential involvement of MEDLE-2 in the invasion of host cells.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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