Identification of Genovariants of Yersinia pestis Strains Belonging to Main Subspecies Using PCR with Hybridization-Fluorescent Registration of Results

Abstract

For the first time ever developed has been the method of identification of genovariants in Yersinia pestis strains belonging to the main subspecies using PCR with hybridization-fluorescent registration of results. A complex of DNA-targets, the most of which are newly detected ones, have been utilized to differentiate between genovariants of antique (0.ANT, 1.ANT, 2.ANT, 4.ANT), medievalis (2.MED, 2.MED0), and orientalis (1.ORI) biovars. Application of these targets in PCR with hybridization-fluorescent registration of results provides for a rapid, effective and reliable classifying of the strains of the genovariants, circulating in various geographical regions. Efficacy of the method has been validated via analysis of 110 Y. pestis strains, isolated in the territory of the Russian Federation, neighboring countries and beyond, including 37 strains of antique, 53 strains of medievalis, and 20 strains of orientalis biovars.

Keywords

• возбудитель чумы
• основной подвид
• геноварианты
• пцр с гибридизационно-флуоресцентным учетом результатов
• plague agent
• the main subspecies
• genovariants
• pcr with hybridization-fluorescent registration of results