Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Luo Yuan; Tseng Ming Lun; Vyas Sunil; Hsieh Ting-Yu; Wu Jui-Ching; Chen Shang-Yang; Peng Hsiao-Fang; Su Vin-Cent; Huang Tzu-Ting; Kuo Hsin Yu; Chu Cheng Hung; Chen Mu Ku; Chen Jia-Wern; Chen Yu-Chun; Huang Kuang-Yuh; Kuan Chieh-Hsiung; Shi Xu; Misawa Hiroaki; Tsai Din Ping

doi:10.1515/nanoph-2021-0748

Nanophotonics (Feb 2022)

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Luo Yuan,
Tseng Ming Lun,
Vyas Sunil,
Hsieh Ting-Yu,
Wu Jui-Ching,
Chen Shang-Yang,
Peng Hsiao-Fang,
Su Vin-Cent,
Huang Tzu-Ting,
Kuo Hsin Yu,
Chu Cheng Hung,
Chen Mu Ku,
Chen Jia-Wern,
Chen Yu-Chun,
Huang Kuang-Yuh,
Kuan Chieh-Hsiung,
Shi Xu,
Misawa Hiroaki,
Tsai Din Ping

Affiliations

Luo Yuan: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Tseng Ming Lun: Institute of Electronics, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, ROC
Vyas Sunil: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Hsieh Ting-Yu: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Wu Jui-Ching: Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, No. 1., Chang-Te St., Taipei, 100, Taiwan, ROC
Chen Shang-Yang: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Peng Hsiao-Fang: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Su Vin-Cent: Electrical Engineering, National United University, No. 2, Lienda, Miaoli, 36003, Taiwan, ROC
Huang Tzu-Ting: Mechanical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan, ROC
Kuo Hsin Yu: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Chu Cheng Hung: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Chen Mu Ku: Department of Electrical Engineering, City University of Hong Kong, Kowloon, Hong Kong
Chen Jia-Wern: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Chen Yu-Chun: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Huang Kuang-Yuh: Mechanical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan, ROC
Kuan Chieh-Hsiung: National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC
Shi Xu: Hokkaido University, Sapporo, Hokkaido, Japan
Misawa Hiroaki: Hokkaido University, Sapporo, Hokkaido, Japan
Tsai Din Ping: Department of Electrical Engineering, The Hong Kong Polytechnic University, City University of Hong Kong, Kowloon, Hong Kong

DOI: https://doi.org/10.1515/nanoph-2021-0748
Journal volume & issue: Vol. 11, no. 9
pp. 1949 – 1959

Abstract

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Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Published in Nanophotonics

ISSN: 2192-8606 (Print); 2192-8614 (Online)
Publisher: De Gruyter
Country of publisher: Germany
LCC subjects: Science: Physics
Website: https://www.degruyter.com/journal/key/nanoph/html#submit

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