Molecules (Nov 2020)

Anti-Melanogenic Effects of Ethanol Extracts of the Leaves and Roots of <i>Patrinia villosa</i> (Thunb.) Juss through Their Inhibition of CREB and Induction of ERK and Autophagy

  • Deok Jeong,
  • Sang Hee Park,
  • Min-Ha Kim,
  • Sarah Lee,
  • Yoon Kyung Cho,
  • You Ah Kim,
  • Byoung Jun Park,
  • Jongsung Lee,
  • Hakhee Kang,
  • Jae Youl Cho

DOI
https://doi.org/10.3390/molecules25225375
Journal volume & issue
Vol. 25, no. 22
p. 5375

Abstract

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Patrinia villosa (Thunb.) Juss is a traditional herb commonly used in East Asia including Korea, Japan, and China. It has been administered to reduce and treat inflammation in Donguibogam, Korea. The mechanism for its anti-inflammatory effects has already been reported. In this study, we confirmed the efficacy of Patrinia villosa (Thunb.) Juss ethanol extract (Pv-EE) for inducing autophagy and investigate its anti-melanogenic properties. Melanin secretion and content were investigated using cells from the melanoma cell line B16F10. Pv-EE inhibited melanin in melanogenesis induced by α-melanocyte-stimulating hormone (α-MSH). The mechanism of inhibition of Pv-EE was confirmed by suppressing the mRNA of microphthalmia-associated transcription factor (MITF), decreasing the phosphorylation level of CREB, and increasing the phosphorylation of ERK. Finally, it was confirmed that Pv-EE induces autophagy through the autophagy markers LC3B and p62, and that the anti-melanogenic effect of Pv-EE is inhibited by the autophagy inhibitor 3-methyl adenine (3-MA). These results suggest that Pv-EE may be used as a skin protectant due to its anti-melanin properties including autophagy.

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