Generation of induced pluripotent stem cell line NIMHi010-A from dermal fibroblast cells of a healthy individual

Suravi Sasmita Dash; Gautham Arunachal; Madhura Milind Nimonkar; Seena Vengalil; Saraswati Nashi; Ghati K. Chetan; Vijay Kumar Boddu; Atchayaram Nalini; Yogananda S. Markandeya

Stem Cell Research (Apr 2024)

Generation of induced pluripotent stem cell line NIMHi010-A from dermal fibroblast cells of a healthy individual

Suravi Sasmita Dash,
Gautham Arunachal,
Madhura Milind Nimonkar,
Seena Vengalil,
Saraswati Nashi,
Ghati K. Chetan,
Vijay Kumar Boddu,
Atchayaram Nalini,
Yogananda S. Markandeya

Affiliations

Suravi Sasmita Dash: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Gautham Arunachal: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Madhura Milind Nimonkar: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Seena Vengalil: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Saraswati Nashi: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Ghati K. Chetan: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Vijay Kumar Boddu: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Atchayaram Nalini: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Yogananda S. Markandeya: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India; Corresponding author.

Journal volume & issue: Vol. 76
p. 103355

Abstract

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In this study, we have established human induced pluripotent stem cell (hiPSC) line, NIMHi010-A of a 42-year-old healthy donor. The iPSC line was generated from human dermal fibroblasts using Sendai viruses carrying reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The generated hiPSC line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line will serve as a healthy control model for physiological processes and drug screening of Asian origin from Indian population.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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