The use of an immunoelectron microscope-decoration technique to observe serological relationship of carlaviruses

Abstract

An immunoelectron microscope-decoration technique was used to define serological relationships among carlaviruses (5 viruses and 9 antisera). The degree of relationship among these viruses was assessed on the basis of the intensity of antibody coating when the viruses were decorated with heterologous antibodies. A relationship was observed among PVM, PVS, CLV, CVB, LilMV, HoLV, HoMV and between WPSV and LilMV. PopMV was not related serologically to any of these viruses. The PVS particles were occasionally surrounded by an external spiral of an unidentified protein material.