High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena

Abstract

A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 zeatin, 0.1 mg dm-3 indoleacetic acid and 10 g dm-3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0-2.0 mg dm-3 gibberellic acid and 4.0-8.0 mg dm-3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.

Keywords

• agno3
• eggplant
• flow cytometry analysis
• rapd
• ssr