Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Åsa Fex Svenningsen; Morten Meyer; Tanja Maria Michel

Stem Cell Research (Dec 2020)

Generation of autism spectrum disorder patient-derived iPSC line SDUKIi004-A

Morad Kamand,
Mirolyuba Ilieva,
Sheena Louise Forsberg,
Mads Thomassen,
Åsa Fex Svenningsen,
Morten Meyer,
Tanja Maria Michel

Affiliations

Morad Kamand: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark; Corresponding authors at: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark (M. Kamand). Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark (T.M. Michel).
Mirolyuba Ilieva: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark
Sheena Louise Forsberg: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark
Mads Thomassen: Department of Clinical Research, Department of Human Genetics, Odense University Hospital, Denmark
Åsa Fex Svenningsen: Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Brain Research - Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark
Morten Meyer: Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Brain Research - Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Department of Neurology, Odense University Hospital, Denmark
Tanja Maria Michel: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark; Brain Research - Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark; Corresponding authors at: Department of Clinical Research, Department of Psychiatry, University of Southern Denmark, Odense, Denmark (M. Kamand). Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark (T.M. Michel).

Journal volume & issue: Vol. 49
p. 102038

Abstract

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Autism is a heterogeneous neurodevelopmental disorder defined by deficits in socialization, communication, and patterns of behavior. Using stem cells to model brain disorders may yield new understanding about the underlying neuropathological processes and could prove essential for drug development. We present here a new human induced pluripotent stem cell (iPSC) line (SDUKIi004-A) generated from skin fibroblasts derived from a 21-year old male patient diagnosed with Pervasive Developmental Disorder-Not Otherwise Specified (PDD-NOS) (“FYNEN-cohort”). Reprogramming of the fibroblasts was accomplished using integration-free episomal plasmids. Characterization validated the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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