SUPEROXIDE AND PEROXYNITRITE PRODUCTION IN GASTRIC MUCOSA OF RATS UNDER COMBINED NITRATE-FLUORIDE INTOXICATION

Abstract

The aim of the research is to study influence of combined nitrate-fluoride intoxication on the production of peroxynitrite (ONOO-) and superoxide anion radical (O2•-) in gastric mucosa of rats. Material and methods. We carried out experiments on 52 mature rats. Intoxication was modeled by intragastric administration of sodium fluoride and nitrate in a dose of 10 mg/kg and 500 mg/kg respectively. Total production of O2•-, ONOO- and concentration of thiobarbituric acid reactants (TBA-reactants) was determined in 10% homogenate of gastric mucosa. Results. We observed the highest ONOO- production from fluoride intoxication, while O2•- production was the highest under combined intoxication. The TBA-reactant concentration was the highest in fluoride intoxication group. Conclusion. Combined nitrate-fluoride intoxication intensifies lipid peroxidation by increased production of both O2•- and ONOO-.

Keywords

• nitrates
• fluorides
• peroxynitrite
• reactive oxygen species