Nature Communications (Nov 2022)
Induction of cross-neutralizing antibodies by a permuted hepatitis C virus glycoprotein nanoparticle vaccine candidate
- Kwinten Sliepen,
- Laura Radić,
- Joan Capella-Pujol,
- Yasunori Watanabe,
- Ian Zon,
- Ana Chumbe,
- Wen-Hsin Lee,
- Marlon de Gast,
- Jelle Koopsen,
- Sylvie Koekkoek,
- Iván del Moral-Sánchez,
- Philip J. M. Brouwer,
- Rashmi Ravichandran,
- Gabriel Ozorowski,
- Neil P. King,
- Andrew B. Ward,
- Marit J. van Gils,
- Max Crispin,
- Janke Schinkel,
- Rogier W. Sanders
Affiliations
- Kwinten Sliepen
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Laura Radić
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Joan Capella-Pujol
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Yasunori Watanabe
- School of Biological Sciences, University of Southampton
- Ian Zon
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Ana Chumbe
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Wen-Hsin Lee
- Department of Structural Biology and Computational Biology, The Scripps Research Institute
- Marlon de Gast
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Jelle Koopsen
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Sylvie Koekkoek
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Iván del Moral-Sánchez
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Philip J. M. Brouwer
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Rashmi Ravichandran
- Department of Biochemistry, University of Washington
- Gabriel Ozorowski
- Department of Structural Biology and Computational Biology, The Scripps Research Institute
- Neil P. King
- Department of Biochemistry, University of Washington
- Andrew B. Ward
- Department of Structural Biology and Computational Biology, The Scripps Research Institute
- Marit J. van Gils
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Max Crispin
- School of Biological Sciences, University of Southampton
- Janke Schinkel
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- Rogier W. Sanders
- Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Laboratory of Experimental Virology
- DOI
- https://doi.org/10.1038/s41467-022-34961-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
E1E2 spike on the hepatitis C virion is an important target for vaccine design. Here, the authors permute the subunits to generate E2E1 immunogens and show that mosaic nanoparticles displaying different E2E1 antigens elicit cross-neutralizing antibodies in rabbits.
