Redox Behavior of Anticancer Chalcone on a Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

Abstract

The interaction of anticancer chalcone [AMC, 1-(4'-aminophenyl)-3-(4-N,Ndimethylphenyl)- 2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC –DNA intercalation. A binding constant (K) with a value of 6.15 × 105 M-1 was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (Db and Du), heterogeneous electron transfer rate constant (ko) and electron affinity (A) were also calculated from electrochemical data.

Keywords

• DNA
• hypochromism
• bathochromic shift
• binding constant.