Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis

Anna Shcherbina; Jacqueline Larouche; Paula Fraczek; Benjamin A. Yang; Lemuel A. Brown; James F. Markworth; Carolina H. Chung; Mehwish Khaliq; Kanishka de Silva; Jeongmoon J. Choi; Mohammad Fallahi-Sichani; Sriram Chandrasekaran; Young C. Jang; Susan V. Brooks; Carlos A. Aguilar

Cell Reports (Jul 2020)

Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis

Anna Shcherbina,
Jacqueline Larouche,
Paula Fraczek,
Benjamin A. Yang,
Lemuel A. Brown,
James F. Markworth,
Carolina H. Chung,
Mehwish Khaliq,
Kanishka de Silva,
Jeongmoon J. Choi,
Mohammad Fallahi-Sichani,
Sriram Chandrasekaran,
Young C. Jang,
Susan V. Brooks,
Carlos A. Aguilar

Affiliations

Anna Shcherbina: Department of Biomedical Informatics, Stanford University, Palo Alto, CA 94305, USA
Jacqueline Larouche: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
Paula Fraczek: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
Benjamin A. Yang: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
Lemuel A. Brown: Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA
James F. Markworth: Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA
Carolina H. Chung: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Mehwish Khaliq: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Program in Cancer Biology, University of Michigan, Ann Arbor, MI 48109, USA
Kanishka de Silva: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA
Jeongmoon J. Choi: Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Mohammad Fallahi-Sichani: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22903, USA
Sriram Chandrasekaran: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Young C. Jang: Parker H. Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Susan V. Brooks: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA
Carlos A. Aguilar: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Biointerfaces Institute, University of Michigan, Ann Arbor, MI 48109, USA; Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA; Corresponding author

Journal volume & issue: Vol. 32, no. 4
p. 107964

Abstract

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Summary: During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility and quality of life. The repair of skeletal muscle is regulated by tissue-resident stem cells called satellite cells (or muscle stem cells [MuSCs]), but in aging, MuSCs decrease in numbers and regenerative capacity. The transcriptional networks and epigenetic changes that confer diminished regenerative function in MuSCs as a result of natural aging are only partially understood. Herein, we use an integrative genomics approach to profile MuSCs from young and aged animals before and after injury. Integration of these datasets reveals aging impacts multiple regulatory changes through significant differences in gene expression, metabolic flux, chromatin accessibility, and patterns of transcription factor (TF) binding activities. Collectively, these datasets facilitate a deeper understanding of the regulation tissue-resident stem cells use during aging and healing.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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