Expression of ATF3, FOXF1 and CENPU genes in U87 glioma cells with suppressed function of ERN1 signaling enzyme upon glutamine and glucose deprivation

Abstract

It was shown that the expression level of transcription factors ATF3 and FOXF1 genes is increased, but tumor suppressor gene CENPU is strongly decreased in U87 glioma cell with functionally active the sensor and signaling enzyme ERN1 upon glutamine deprivation condition. At the same time, in glioma cells with suppressed activity of ERN1 the expression level of FOXF1 gene does not change significantly upon glutamine deprivation, but ATF3 gene is increased and CENPU is decreased; however, the inhibition of ERN1 significantly enhances the sensitivity of CENPU gene to glutamine deprivation. The expression of FOXF1 gene is decreased upon glucose deprivation condition only in cells with functionally active ERN1. Inhibition of ERN1 down-regulates the expression levels of ATF3 and FOXF1 genes as well as up-regulates CENPU gene expression in glioma cells upon standard growing condition. Thus, the ATF3, FOXF1 and CENPU genes are related to the regulation of cell proliferation in glioma cells and are sensitive to glutamine and glucose deprivation in dependence of ERN1 enzyme function.

Keywords

• gene expressions, ATF3, FOXF1, CENPU, glioma cells, glucose and glutamine deprivations, ERN1