Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies

Tomoko Honda; Sumiko Gomi; Daisuke Yamane; Fumihiko Yasui; Takuya Yamamoto; Tsubasa Munakata; Yasushi Itoh; Kazumasa Ogasawara; Takahiro Sanada; Kenzaburo Yamaji; Yasuhiro Yasutomi; Kyoko Tsukiyama-Kohara; Michinori Kohara

doi:10.1128/mSphere.01342-20

mSphere (Jun 2021)

Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies

Tomoko Honda,
Sumiko Gomi,
Daisuke Yamane,
Fumihiko Yasui,
Takuya Yamamoto,
Tsubasa Munakata,
Yasushi Itoh,
Kazumasa Ogasawara,
Takahiro Sanada,
Kenzaburo Yamaji,
Yasuhiro Yasutomi,
Kyoko Tsukiyama-Kohara,
Michinori Kohara

Affiliations

Tomoko Honda: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Sumiko Gomi: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Daisuke Yamane: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Fumihiko Yasui: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Takuya Yamamoto: Laboratory of Immunosenescence, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan
Tsubasa Munakata: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Yasushi Itoh: ORCiD; Division of Pathogenesis and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Setatsukinowa, Otsu, Shiga, Japan
Kazumasa Ogasawara: Division of Pathogenesis and Disease Regulation, Department of Pathology, Shiga University of Medical Science, Setatsukinowa, Otsu, Shiga, Japan
Takahiro Sanada: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Kenzaburo Yamaji: Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Yasuhiro Yasutomi: Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Japan
Kyoko Tsukiyama-Kohara: Transboundary Animal Diseases Centre, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Kagoshima, Japan
Michinori Kohara: ORCiD; Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

DOI: https://doi.org/10.1128/mSphere.01342-20
Journal volume & issue: Vol. 6, no. 3

Abstract

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Influenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of sera.

Published in mSphere

ISSN: 2379-5042 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msphere

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