IN VITRO MICROPROPAGATION OF ZANTEDESCHIA ELLIOTTIANA PLANTS

Abstract

A set of experiments was done on Zantedeschia elliottiana tubers at Tissue Culture Laboratory, Faculty of Agric. Saba Basha, Alex. Univ., Egypt; during the years of 2021 and 2022 to achieve an optimal and reliable system for its micropropagation. At least one tuber eye derived from (1 cm2) tuber piece was used as initial explants. The explants were drenched in L-ascorbic acid solution(10 mg/l). The explants were cultured on initiation media (MS medium) complement with auxin: 1-naphthaleneacetic acid (NAA) and cytokinin: N6-benzyladenine (BA) [0 to 2 mg/l]. On multiplication stage, adventitious shoots were put in MS media complement with BA (0.5 to 5 mg/l), kinetin: KIN (0.5 to 5 mg/l), with NAA (0.5 to 2 mg/l). The effect of BA is more than KIN in multiplication stage, taller shoots and large number of adventitious shoots were produced after addition of BA to culture medium. Top efficient combination of the studied cytokinins and auxins on multiplication stage was obtained from the addition of 3 mg/l BA and NAA at 2.00 mg/l, which led to the highest increase in number of shoots, leaflets and root numbers/propagule. Rooting took place on MS media complement with auxin: indole-3-butyric (IBA) and NAA at (0.5 to 2 mg/l). The most plentiful and the longest roots were obtained from explants cultured on MS medium complement with IBA at 1.0 mg/l. The highest rooting percentage 100% was obtained after the addition of NAA at 0.5 mg/l to culture media. Acclamation percent reached up to 90% under greenhouse conditions

Keywords

• zantedeschia elliottiana
• in vitro propagation
• 1-naphthaleneacetic acid (naa)
• n6-benzyladenine
• indole-3-butyric acid (iba)
• kinetin (kin)