Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism

Abstract

Objective To investigate the effects and mechanism of chlorogenic acid (CGA) on the proliferation and apoptosis of human glioma cell line U251. Methods Human glioma U251 cells were treated with different concentrations of chlorogenic acid for 48h,then CCK-8 was used to detect the inhibitory rate of cell growth. Annexin V-FITC/PI staining assay was used to detect the apoptosis. RT-PCR and Western blot were used to measure the mRNA and protein levels of p53,Livin,Bcl-2 and Bax,and the protein level of Caspase-3 was detected by Western blot. Results Different concentrations of chlorogenic acid significantly inhibited the proliferation of U251 cells and promoted the apoptosis. Chlorogenic acid could up-regulate the expression of p53,Bax and down-regulate the expression of Livin,Bcl-2 at mRNA and protein levels,and increase the protein level of Caspase-3. Conclusion CGA could inhibit the proliferation and induce the apoptosis of U251 cells via up-regulating p53 and Bax expression,down-regulating Livin and Bcl-2 expression,and activating Caspase-3 protein.

Keywords

• chlorogenic acid
• u251 cells
• apoptosis
• p53
• livin