Rapid molecular detection of root-knot nematode Meloidogyne incognita in coffee (Coffea arabica L.) roots

Abstract

Coffee is one of the most relevant agricultural products globally, due to its significant economic, social and environmental impact, especially in producing countries such as Peru. In particular, the San Martín region stands out for its production of 83425 tons of parchment coffee in an area of ​​81000 hectares. Root-knot nematodes, especially of the genus Meloidogyne, are a significant threat to coffee cultivation, affecting plant water and nutrient uptake. Accurate and rapid detection of these nematodes is crucial for their effective control. The objective was based on the rapid molecular detection of the root-knot nematode Meloidogyne incognita in coffee roots by PCR with the specific primers MI-F and MI-R to amplify a band of 999 bp. The isolation of DNA allowed concentrations between 59.1 to 39.4 ng/µL and absorbance ranges from 1.92 to 2.00, being determined as good quality DNA. This study focuses on the molecular detection of M. incognita in coffee roots. These results allow accurate and rapid detection of nematodes in coffee, and thus be able to control this pathogen in time.

Keywords

• DNA
• molecular biology
• specific primers
• phytoparasites