Generation of a human induced pluripotent stem cell line (OGIi001) from peripheral blood mononuclear cells of a healthy male donor

Hanmeng Zhang; Laurence Daheron; Rodrigo Cerna-Chavez; Emily M. Place; Rachel M. Huckfeldt; Eric A. Pierce; Marcela Garita-Hernandez

Stem Cell Research (Feb 2024)

Generation of a human induced pluripotent stem cell line (OGIi001) from peripheral blood mononuclear cells of a healthy male donor

Hanmeng Zhang,
Laurence Daheron,
Rodrigo Cerna-Chavez,
Emily M. Place,
Rachel M. Huckfeldt,
Eric A. Pierce,
Marcela Garita-Hernandez

Affiliations

Hanmeng Zhang: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA
Laurence Daheron: Harvard Stem Cell Institute, Harvard University, Cambridge, MA, 02138, USA
Rodrigo Cerna-Chavez: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA
Emily M. Place: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA
Rachel M. Huckfeldt: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA
Eric A. Pierce: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA
Marcela Garita-Hernandez: Ocular Genomics Institute, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA, 02114, USA; Corresponding author.

Journal volume & issue: Vol. 74
p. 103280

Abstract

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We have successfully derived a novel human induced pluripotent stem cell (hiPSC) line using non-integrative Sendai virus. This hiPSC line was generated from a healthy male adult donor, aged 55, and subjected to thorough characterization and extensive quality control. The analysis confirmed the expression of undifferentiated stem cell markers, demonstrated the ability to differentiate into the three germ layers, and revealed the absence of any chromosomal abnormalities.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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