Nature Communications (May 2020)
Photoactivatable Cre recombinase 3.0 for in vivo mouse applications
- Kumi Morikawa,
- Kazuhiro Furuhashi,
- Carmen de Sena-Tomas,
- Alvaro L. Garcia-Garcia,
- Ramsey Bekdash,
- Alison D. Klein,
- Nicholas Gallerani,
- Hannah E. Yamamoto,
- Seon-Hye E. Park,
- Grant S. Collins,
- Fuun Kawano,
- Moritoshi Sato,
- Chyuan-Sheng Lin,
- Kimara L. Targoff,
- Edmund Au,
- Michael C. Salling,
- Masayuki Yazawa
Affiliations
- Kumi Morikawa
- Columbia Stem Cell Initiative, Columbia University
- Kazuhiro Furuhashi
- Columbia Stem Cell Initiative, Columbia University
- Carmen de Sena-Tomas
- Columbia Stem Cell Initiative, Columbia University
- Alvaro L. Garcia-Garcia
- Department of Psychiatry, Division of Systems Neuroscience, New York State Psychiatric Institute, Columbia University
- Ramsey Bekdash
- Columbia Stem Cell Initiative, Columbia University
- Alison D. Klein
- Columbia Stem Cell Initiative, Columbia University
- Nicholas Gallerani
- Columbia Stem Cell Initiative, Columbia University
- Hannah E. Yamamoto
- Columbia Stem Cell Initiative, Columbia University
- Seon-Hye E. Park
- Columbia Stem Cell Initiative, Columbia University
- Grant S. Collins
- Department of Cell Biology and Anatomy, Louisiana State University Health Sciences Center
- Fuun Kawano
- Columbia Stem Cell Initiative, Columbia University
- Moritoshi Sato
- Graduate School of Arts and Sciences, The University of Tokyo
- Chyuan-Sheng Lin
- Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University
- Kimara L. Targoff
- Columbia Stem Cell Initiative, Columbia University
- Edmund Au
- Columbia Stem Cell Initiative, Columbia University
- Michael C. Salling
- Department of Cell Biology and Anatomy, Louisiana State University Health Sciences Center
- Masayuki Yazawa
- Columbia Stem Cell Initiative, Columbia University
- DOI
- https://doi.org/10.1038/s41467-020-16030-0
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Previous versions of photoactivatable Cre recombinase (PA-Cre) suffered from unintentional recombination in dark conditions. Here, the authors develop an improved version of PA-Cre, called PA-Cre 3.0, which shows reduced leakiness and improved efficiency upon activation, and make mouse lines that express PA-Cre 3.0 conditionally.
