Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Ruidong Ni; Suzeeta Bhandari; Perry R. Mitchell; Gabriela Suarez; Neel B. Patel; Kara Lamb; Kirpal S. Bisht; David J. Merkler

doi:10.3390/molecules26092543

Molecules (Apr 2021)

Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Ruidong Ni,
Suzeeta Bhandari,
Perry R. Mitchell,
Gabriela Suarez,
Neel B. Patel,
Kara Lamb,
Kirpal S. Bisht,
David J. Merkler

Affiliations

Ruidong Ni: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Suzeeta Bhandari: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Perry R. Mitchell: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Gabriela Suarez: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Neel B. Patel: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Kara Lamb: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
Kirpal S. Bisht: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA
David J. Merkler: Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA

DOI: https://doi.org/10.3390/molecules26092543
Journal volume & issue: Vol. 26, no. 9
p. 2543

Abstract

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Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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