Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2

Jiangshan Wang; Andres Dextre; Ana Pascual-Garrigos; Josiah Levi Davidson; Murali K. Maruthamuthu; Darby McChesney; Jordan Seville; Mohit S. Verma

MethodsX (Jan 2021)

Fabrication of a paper-based colorimetric molecular test for SARS-CoV-2

Jiangshan Wang,
Andres Dextre,
Ana Pascual-Garrigos,
Josiah Levi Davidson,
Murali K. Maruthamuthu,
Darby McChesney,
Jordan Seville,
Mohit S. Verma

Affiliations

Jiangshan Wang: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA
Andres Dextre: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA
Ana Pascual-Garrigos: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA
Josiah Levi Davidson: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA
Murali K. Maruthamuthu: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA
Darby McChesney: PortaScience, Inc., Moorestown, NJ 08057, USA
Jordan Seville: PortaScience, Inc., Moorestown, NJ 08057, USA
Mohit S. Verma: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA; Corresponding author at: Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.

Journal volume & issue: Vol. 8
p. 101586

Abstract

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The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused unprecedented damage to the global economy. Diagnostic testing is a key factor in limiting virus transmission and safeguarding public health. We present the fabrication process of a paper-based device that uses reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect SARS-CoV-2 in complex matrices by providing a colorimetric response apparent to the naked eye. Because of LAMP's functionality, this device just requires a simple heat source (e.g., water bath, incubator), it can be deployed in resource-constrained areas and can be used as a supplement to current point-of-care (POC) and community testing procedures. Since the test is based on nucleic acids, the testing platform itself lends to further applications including food safety monitoring, animal diagnostics, etc. simply by changing the specific primers. • We developed a platform capable of on-paper detection of SARS-CoV-2 using colorimetric reporters that produce responses visible to the naked eye. • The platform is easily reconfigurable to target different pathogens by changing the primer sets, and multiplexing is possible by adding additional reaction sites to the device.

Paper-based colorimetric LAMP

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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