EEEECT OF KINETIN ON INITIATION AND MULTIPLICATION OF SHOOT TIPS AND NODES OF CARNATION IN VITRO

Abstract

Shoot tips and nodes of two carnation cultivars "Jeanne Dionis Blano" a white flower cv. and "Marie Chabaud Jaune" a yellow flower cv. taken from the field were planted on MS medium supplemented with kinetin at (0.0 , 2.0 , 4.0, 6.0 , 8.0) ml/L. Shoots of "Jeanne Dionis Blano" produced in vitro were rooted on half strength MS medium supplemented with IAA . Data shows that shoot tip response were 100% for in vitro culture . There is no significant differences on shoot numbers between the two cultivars in multiplication stage, shoot tips planting on MS medium supplemented with 8.0mg/L Kin gave the highest number 14.95 shoot/explant in multiplication stage and gave 16.90 shoot/explant for white cv., no significant differences between cultivars from culture node of the two cultivars at the multiplication stage highest shoots number 6.07 shoot/explant were obtained from the node culture on MS medium at 2.0 mg/L Kin and the highest shoot number 12.70 shoot were obtained from shoot tip culture of yellow cv. on MS medium supplemented with 8.0 mg/L Kin, shoots which produced in vitro for white cv. gave the highest rooting 50% with the highest root number 1.75 root/shoot when culture on half strength MS medium supplemented with 1.5 mg /L IAA . All the plantlets which produced in vitro acclimatized in laboratory then transferred to the greenhouse to grow normally.