Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease

Yuwen Cao; Wotu Tian; Li Cao; WenLu Lv; Lan Zheng; Xinghua Luan

Stem Cell Research (Dec 2022)

Generation of an induced pluripotent stem cell JTUi005-A from a patient with neuronal intranuclear inclusion disease

Yuwen Cao,
Wotu Tian,
Li Cao,
WenLu Lv,
Lan Zheng,
Xinghua Luan

Affiliations

Yuwen Cao: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
Wotu Tian: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
Li Cao: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
WenLu Lv: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
Lan Zheng: Department of Neurology, Minhang Hospital, Fudan University, Shanghai, China; Corresponding authors at: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (X. Luan).
Xinghua Luan: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China; Department of Neurology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Corresponding authors at: Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China (X. Luan).

Journal volume & issue: Vol. 65
p. 102938

Abstract

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Neuronal intranuclear inclusion disease is a neurodegenerative disease caused by expansion of GGC repeats in the 5′ untranslated region (5′ UTR) of NOTCH2NLC. An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells of a 55-year-old male patient by expressing a defined set of reprogramming factors (OCT4, SOX2, NANOG, LIN28, c-MYC and KLF4) carried on episomal vectors, and was validated for stem cell-like pluripotency, normal karyotype and capability of in vivo differentiation into three germ layers. The NIID-iPSC line serves as a promising tool for further research into pathogenic mechanism and potential therapeutic targets.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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