Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Sai Li; Michael R. Wasserman; Olga Yurieva; Lu Bai; Michael E. O’Donnell; Shixin Liu

doi:10.1038/s41467-022-32657-7

Nature Communications (Aug 2022)

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Sai Li,
Michael R. Wasserman,
Olga Yurieva,
Lu Bai,
Michael E. O’Donnell,
Shixin Liu

Affiliations

Sai Li: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University
Michael R. Wasserman: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University
Olga Yurieva: Laboratory of DNA Replication, The Rockefeller University
Lu Bai: Center for Eukaryotic Gene Regulation, The Pennsylvania State University
Michael E. O’Donnell: Laboratory of DNA Replication, The Rockefeller University
Shixin Liu: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University

DOI: https://doi.org/10.1038/s41467-022-32657-7
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions and recruit replicative helicases to form a pre-replication complex independent of the DNA sequence.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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