RESULTS OF DETERMINING THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI USING REAL-TIME PCR TESTING AND BACTERIOLOGICAL METHOD

Abstract

Aim. To compare the species composition of lactobacilli in women of reproductive age using real time PCR on urogenital samples before and after culturing microorganisms in Blood-Serum-Y^ast extract-Agar (BSYA). Materials and methods. Using real time PCR, we have examined the species composition of vaginal lactobacilli in 25 healthy women of reproductive age. Samples of urogenital swabs (endocervical and vaginal swabs) were studied. Species identification has been carried out twice: in the native clinical material and in the samples received after using microorganism culture technique in BSYA. Results. After culturing vaginal microorganisms in BSYA, L. iners predominance was found only in 2 cases (8%). At the same time, when using native clinical material this species predominance was discovered in 10 samples (40%). Conclusion. When culturing lactobacilli in BSYA, the growth of L. iners is stunted compared to other species of vaginal lactobacilli. The use of cultural testing turned out to be ineffective in case of predominance of L. iners, the species associated with an increased risk of vaginal dysbiosis.

Keywords

• lacrobacillus crispatus
• lactobacillus iners
• lactobacillus gasseri
• lactobacillus jensenii
• vaginal microbiocenosis
• vaginal lactobacilli
• vaginal dysbiosis
• normocenosis
• lacrobacillus crispatus
• lactobacillus iners
• lactobacillus gasseri
• lactobacillus jensenii
• realtime pcr