The Effect of Plant Growth Regulators on Shoot Proliferation and Rooting of Crataegus Pseudohetrophylla Pojark. Via in Vitro Culture

Abstract

Crataegus is a tree species from Rosaceae family with medicinal, ornamental and commercial utilizations. Effect of different concentrations of plant growth regulators on shoot proliferation of Crataegus pseudohetrophylla Pojark. via in vitro culture was studied using single node explants. The grown shoots were transferred to MS medium supplemented with different concentrations of cytokinins (BAP, KIN, 2iP and Zt) in combination with NAA in 30 treatments, with 3 replications and each replication included 5 explants. Data were recorded after 3 subcultures. For rooting, basal end of shoots were dipped into 300mg/l of IBA at different durations (0, 5, 10, 20, 30, 40, 50 and 60 minutes), then the dipped shoots were cultured on hormone free 1/2 VS medium. The results of analysis of variance showed that there were significant differences in all of the traits. Results indicated that the highest number of shoots (39.33) was obtained on medium containing 8 mg / l BAP plus 2 mg / l NAA. The highest shoot length (4.67 cm) and leaf size (3.73 cm) was achieved on 7 mg / l BAP plus 2 mg / l NAA and 2 mg / l Zt plus 1 mg / l NAA, respectively. The highest rate of rooting (33.33%) and root number (4n) were induced on shoots dipped for 40 minutes in 300 mg / l IBA.

Keywords

• BAP
• Cytokinin
• Shoots
• Single node culture
• NAA