L-Ascorbic acid affect the DNA methytransferase expression in Mouse Embryonic Fibroblasts

Abstract

Introduction: Induced pluripotent stem cells (iPSCs) can be generated from different source of cells with different efficiencies. Two DNA methyltransferases DNMT1 and DNMT3A have been shown to regulate epigenetically the gene expression involved in cell viability and reprogramming. L-ascorbic acid (L-AA) is a chemical factor that can accelerate reprogramming. Here, we sought to investigate the effect of L-AA on DNMT1 and DNMT3A expressions. Materials and Methods: First, mouse embryonic fibroblasts at passage 3 were cultured in the presence of 10 µg/ml L-AA days for 5 days. Then, DNMT1 and DNMT3A expressions were determined using real-time PCR at days 3 and 5. Results: It was showed that L-AA could enhance DNMT-1 expression which involve in cell viability and decrease the DNMT3A which involve in cell differentiation. Conclusion: The results therefore suggest a new insight into L-AA mechanism impact on reprogramming process.

Keywords

• Induced pluripotent stem cells
• DNA methyltransferases
• DNMT1
• DNMT3A
• reprogramming
• L-ascorbic acid