Zhongguo youzhi (Jul 2022)
辣木籽提取物对高脂血症小鼠血脂及肝脏的影响Effect of Moringa oleifera seed extract on blood lipid and liver in hyperlipidemia mice
Abstract
通过向高脂血症模型小鼠灌胃辣木籽提取物，研究辣木籽提取物对高脂血症小鼠血脂及肝脏的影响。将60 只KM小鼠按体质量随机分为正常组，模型组，辛伐他汀组(1 mg/kg)，辣木籽提取物低(5 mg/kg)、中(10 mg/kg)、高(20 mg/kg)剂量组，每组10 只。除正常组饲喂基础饲料外，其余各组小鼠以高脂饲料连续喂养。实验第6周开始，除正常组和模型组外，其余各组小鼠灌胃给予相应药物，连续3周。实验结束后测定各组小鼠血清HDL-C、LDL-C、 TG、TC水平；剖取肝脏，测定肝脏指数并进行病理形态学观察；采用免疫组化法检测肝脏组织法尼酯X受体（FXR）蛋白的表达水平。结果表明：低、中、高剂量辣木籽提取物可显著降低高脂血症小鼠血清TG、TC及LDL-C水平（p<0.05），极显著升高HDL-C水平（p<0.01），改善小鼠肝脏病理形态，极显著降低小鼠肝脏病理评分（p<0.01）；低剂量辣木籽提取物可显著降低小鼠肝脏指数，低、中剂量辣木籽提取物可显著降低肝脏FXR蛋白的表达（p<0.05）。因此，一定剂量的辣木籽提取物对高脂血症小鼠具有显著的治疗作用，其作用机制可能与下调 FXR蛋白表达有关。 By gavage of Moringa oleifera seed extract to hyperlipidemia mice model, the effects of Moringa oleifera seed extract on blood lipid and liver in hyperlipidaemia mice were studied. Sixty KM mice were randomly divided into control group, model group, simvastatin group (1 mg/kg), Moringa oleifera seed extract low (5 mg/kg), medium (10 mg/kg) and high (20 mg/kg) dose groups according to body mass, 10 mice in each group. Except for the control group fed the basic feed, the other groups were given high-fat diet. At the sixth week, except for the control group and the model group, the mice in other groups were given the drugs correspondingly in serum for three weeks. The HDL-C, LDL-C, TG and TC levels in serum of mice were measured at the end of the experiment. The liver index and pathomorphology were detected and the farnesoid X receptor（FXR） protein expression in liver were determined by immunohistochemistry. The results showed that the low, medium and high dose Moringa oleifera seed extract significantly reduced TG, TC and LDL-C levels(p<0.05) and increased HDL-C levels(p<0.01)in serum of hyperlipidemia mice, significantly improved the pathological morphology of liver, markedly reduce the liver pathology score(p<0.01), low dose Moringa oleifera seed extract could remarkably reduce the liver index(p<0.05), and low and high dose Moringa oleifera seed extract significantly reduced the FXR protein expression in liver (p<0.05). Moringa oleifera seed extract has significant therapeutic effects in hyperlipidemic mice, and its mechanism may be related to the down-regulation of FXR protein expression in liver.
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