In vitro establishment of apexes of plants from field of the Cuban hybrid of Papaya IBP 42-99.

Abstract

The present work was carried out with the objective of developing a protocol for the establishment of shoot tips in vitro of papaya, starting from adult plants in the field, which had 11 months of having planted in field. Plants were selected which are beheaded the apical area and other not, besides the application to foliate of a mixture of growth regulators of 6 BAP and giberelic acid. For the disinfection of the explantes different substances were used, alone or in combination: Sodium Hypochlorite (0.5, 1.0, 1.5, 2.0, 3.0%), Alcohol to 70% and a mixture of antibiotics. The best results were reached when using the Sodium Hypochlorite to 1.0% during 10 minutes, after the previous submersion of the apexes in the mixture of antibiotics for 30 minutes and using buds of 2 months of age, what allowed to achieve 68.5 % establishment. Key word: antibiotics mixture, carica papaya, disinfection,micropropagation