Protective mechanisms of intra- and extracellular polysaccharides from Schizophyllum commune on H2O2-induced oxidative damage of human skin fibroblasts

Wenjing Cheng; Yundong Yang; Jiachan Zhang; Fei qian Di; Luyao Li; Changtao Wang; Meng Li; Dan Zhao; Xiuqin Shi; Tong Huo; Quan An

doi:10.1080/09540105.2023.2203407

Food and Agricultural Immunology (Dec 2023)

Protective mechanisms of intra- and extracellular polysaccharides from Schizophyllum commune on H2O2-induced oxidative damage of human skin fibroblasts

Wenjing Cheng,
Yundong Yang,
Jiachan Zhang,
Fei qian Di,
Luyao Li,
Changtao Wang,
Meng Li,
Dan Zhao,
Xiuqin Shi,
Tong Huo,
Quan An

Affiliations

Wenjing Cheng: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Yundong Yang: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Jiachan Zhang: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Fei qian Di: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Luyao Li: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Changtao Wang: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Meng Li: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Dan Zhao: College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing, People’s Republic of China
Xiuqin Shi: Yunnan Baiyao Group Co., Ltd., Kunming, People’s Republic of China
Tong Huo: Yunnan Baiyao Group Co., Ltd., Kunming, People’s Republic of China
Quan An: Yunnan Baiyao Group Co., Ltd., Kunming, People’s Republic of China

DOI: https://doi.org/10.1080/09540105.2023.2203407
Journal volume & issue: Vol. 34, no. 1

Abstract

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ABSTRACTSchizophyllum commune is a fungus with applications both in medicine and foods, which produces IPS and EPS. However, there is no comparative study on the activity comparison of IPS and EPS and its mechanism of antioxidant effect. In this study, the antioxidant activities of the two polysaccharides were measured in biochemical, cellular and molecular levels, and H2O2 induced oxidative damage model of HSFs was established to explore the protective effects of EPS and IPS. We found IPS and EPS both achieved a good antioxidant effect. They can also improve the cellular levels of SOD and reduce MDA. Insights regarding the molecular mechanism were studied, we found that both EPS and IPS could increase the expression of Nrf2 in Keap1-Nrf2/ARE signaling pathway. At the same time, they could inhibit the expression of the negative feedback gene Keap1 and promote the expression of downstream gene GCLC, Gstt-1, Nqo1 and Ho-1.

Published in Food and Agricultural Immunology

ISSN: 0954-0105 (Print); 1465-3443 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Agriculture: Agriculture (General); Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.tandfonline.com/journals/cfai

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