CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

Jonathan L. Schmid-Burgk; Klara Höning; Thomas S. Ebert; Veit Hornung

doi:10.1038/ncomms12338

Nature Communications (Jul 2016)

CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

Jonathan L. Schmid-Burgk,
Klara Höning,
Thomas S. Ebert,
Veit Hornung

Affiliations

Jonathan L. Schmid-Burgk: Institute of Molecular Medicine, University Hospital, University of Bonn
Klara Höning: Institute of Molecular Medicine, University Hospital, University of Bonn
Thomas S. Ebert: Institute of Molecular Medicine, University Hospital, University of Bonn
Veit Hornung: Institute of Molecular Medicine, University Hospital, University of Bonn

DOI: https://doi.org/10.1038/ncomms12338
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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