脂肪酶Rhizopus chinensis催化水解大豆粉末磷脂制备L-α-甘油磷脂酰乙醇胺

Abstract

为考察脂肪酶Rhizopus chinensis催化水解大豆粉末磷脂制备L-α-甘油磷脂酰乙醇胺（L-α-GPE）的可行性，采用脂肪酶Rhizopus chinensis催化水解大豆粉末磷脂制备L-α-GPE，通过单因素实验考察了pH、大豆粉末磷脂质量浓度、反应温度、CaCl2质量浓度、酶浓度对L-α-GPE得率的影响，并对纯化产品进行了红外光谱和超高效液相色谱电喷雾四极杆飞行时间质谱(UPLC-Q-TOF-MS)结构验证。结果表明：在pH 7，大豆粉末磷脂质量浓度5 mg/mL，大豆粉末磷脂磷酸盐缓冲溶液中添加终质量浓度为2 mg/mL的CaCl2、终浓度为30 U/mL的脂肪酶Rhizopus chinensis，于45 ℃下反应15 h后，L-α-GPE得率为93.8%。产品经分离纯化后得到纯度为99.2%的浅黄色黏稠液体，经结构验证确为L-α-GPE。 To investigate the feasibility of L-α-glycerylphosphorylethanolamine (L-α-GPE) preparation from hydrolysis of soybean phospholipids powder with Rhizopus chinensis as catalyst, L-α-GPE was prepared by Rhizopus chinensis-catalyzed hydrolysis of soybean phospholipids powder. The effects of pH, soybean phospholipid powder mass concentration, reaction temperature, CaCl2 mass concentration and enzyme concentration on the yield of L-α-GPE were investigated by single-factor experiment, and the structure of the purified products were verified by infrared spectroscopy and UPLC-Q-TOF-MS. The results showed that the yield of L-α-GPE was 93.8% under the conditions of addition of final mass concentration of CaCl2 2 mg/mL and final concentration of Rhizopus chinensis 30 U/mL in soybean phospholipids powder mass concentration 5 mg/mL, phosphate buffer solution pH 7， reaction time 15 h, and reaction temperature 45℃. After purification， a light yellow viscous liquid product with a purity of 99.2% was obtained, which was verified as L-α-GPE by structural verification.

Keywords

• 脂肪酶rhizopus chinensis；大豆粉末磷脂；l-α-甘油磷脂酰乙醇胺
• rhizopus chinensis; soybean phospholipids powder; l-α-glycerylphosphorylethanolamine