Low-temperature preservation of sterlet reproductive cells (Acipenser ruthenus Linnaeus, 1758) by the acoustic-mechanical influence method

Abstract

To preserve reproductive cells of rare and endangered species of sturgeon fish using low-temperature cryopreservation methods, acoustic-mechanical impact with the use of piezoactuators was used. As a result of the research, the optimum parameters of signal frequency and time of impact on sturgeon cells were established, at which a high survival rate of sperm after defrosting was observed.Using mathematical modelling, the mechanism of acoustic-mechanical influence was studied, which consists in the wave and mechanical action created by the piezoactuator, which leads to acceleration of the process of diffusion of the cryoprotector inside the cells.For the first time a new methodological approach to low-temperature preservation of fish reproductive cells with the use of acoustic-mechanical impact has been proposed, which makes it possible to preserve genetic material with high survival rate and quality indicators.