Journal of Clinical Medicine (Feb 2022)

Efficacy of Arthroscopic Shavers for the Retrieval and Processing of Connective Tissue Progenitor Cells from Subacromial Bursal Tissue

  • Ian J. Wellington,
  • Benjamin C. Hawthorne,
  • James C. Messina,
  • Matthew R. LeVasseur,
  • Mary Beth McCarthy,
  • Mark P. Cote,
  • Augustus D. Mazzocca

DOI
https://doi.org/10.3390/jcm11051272
Journal volume & issue
Vol. 11, no. 5
p. 1272

Abstract

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The purpose of this study is to determine if arthroscopic shavers can effectively collect and process connective tissue progenitor (CTP) cells from subacromial bursal tissue for utilization in rotator cuff repair augmentation. Subacromial bursal tissue was collected and processed using two arthroscopic shavers, Shaver A and Shaver B, in 10 patients undergoing arthroscopic rotator cuff repair. Each shaver was used in a random order for the same patient. Tissue samples underwent testing for cellular proliferation, cellular concentration, number of colony-forming units (CFU), live/dead assay, fluorescence-activated cells sorting (FACS) analysis, cytokine analysis, and growth factor analysis. Shaver A produced more CFUs compared to Shaver B (210.3 vs. 125.9; p p p p p p p < 0.05). Arthroscopic shavers are capable of harvesting and processing CTP cells from subacromial bursal tissue. Different shavers may produce different yields of viable CTP cells.

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