Biocompatible cdots dual role: Nanophotosensitizers for hypoxic photodynamic therapy and scavenging nanozyme biosensing

Helena M.R. Gonçalves; Susana A.F. Neves; Sabela Rodriguez; Rui F.P. Pereira; Rui Vilarinho; Tânia Moniz; Maria Rangel; J. Agostinho Moreira; Paula Martins-Lopes; Filomena Adega; Frank Davis; Séamus P.J. Higson; Marita A. Cardoso

Biosensors and Bioelectronics: X (Aug 2024)

Biocompatible cdots dual role: Nanophotosensitizers for hypoxic photodynamic therapy and scavenging nanozyme biosensing

Helena M.R. Gonçalves,
Susana A.F. Neves,
Sabela Rodriguez,
Rui F.P. Pereira,
Rui Vilarinho,
Tânia Moniz,
Maria Rangel,
J. Agostinho Moreira,
Paula Martins-Lopes,
Filomena Adega,
Frank Davis,
Séamus P.J. Higson,
Marita A. Cardoso

Affiliations

Helena M.R. Gonçalves: LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal; LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade Do Porto, Rua Do Campo Alegre, s/n, 4169-007, Porto, Portugal; Corresponding author.
Susana A.F. Neves: LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal
Sabela Rodriguez: LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal
Rui F.P. Pereira: Chemistry Department and Chemistry Centre, University of Minho, 4710-057, Braga, Portugal
Rui Vilarinho: IFIMUP, Physics and Astronomy Department of Faculty of Sciences of University of Porto, Porto, Portugal
Tânia Moniz: LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade Do Porto, Rua Do Campo Alegre, s/n, 4169-007, Porto, Portugal; LAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade Do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal
Maria Rangel: LAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, Universidade Do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal
J. Agostinho Moreira: IFIMUP, Physics and Astronomy Department of Faculty of Sciences of University of Porto, Porto, Portugal
Paula Martins-Lopes: DNA & RNA Sensing Lab, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Blocos Laboratoriais Ed, 5000-801, Vila Real, Portugal; BioISI — Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016, Lisboa, Portugal
Filomena Adega: BioISI — Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016, Lisboa, Portugal; CytoGenomics Lab, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal
Frank Davis: Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK
Séamus P.J. Higson: Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK
Marita A. Cardoso: LAQV, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal; DNA & RNA Sensing Lab, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Blocos Laboratoriais Ed, 5000-801, Vila Real, Portugal; BioISI — Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016, Lisboa, Portugal; Corresponding author.

Journal volume & issue: Vol. 19
p. 100499

Abstract

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Within this study we report a non-toxic nanomaterial suitable for Photodynamic Therapy (PDT) under hypoxic conditions. PDT relies on the production of reactive oxygen and nitrogen species that can lead to cancer cells death. Currently, PDT is limited by: the development of efficient photosensitizers that can produce these radicals in situ; and the oxygen level requirement. The produced Carbon Dots (Cdots) successfully destroy human melanoma cancer cells upon 5 min irradiation using 450 nm wavelength due to the in situ production of NO•. As such, this nanophotosensitizer is applicable regardless of the cells molecular oxygen levels. Additionally, this nanomaterial acts as a scavenging nanozyme biosensor allowing to follow-up, in situ, the released NO• concentration, thereby offering a tight control over the NO• concentration in real-time and, its maintenance within the therapeutic window. Hence, this work offers a novel theranostic and NO• scavenging nanozyme biosensing nanoplatform that allows high control for hypoxic-PDT cancer application even in low doses and with 5 min irradiation.

Published in Biosensors and Bioelectronics: X

ISSN: 2590-1370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biosensors-and-bioelectronics-x

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