Green Synthesis and Characterization of Silver Nanoparticles Using Moringa Peregrina and Their Toxicity on MCF-7 and Caco-2 Human Cancer Cells

Abstract

Khaled Saeed Yousef Al Baloushi,1 Annadurai Senthilkumar,1,2 Karthishwaran Kandhan,1 Radhakrishnan Subramanian,1 Jaleel Kizhakkayil,3 Tholkappiyan Ramachandran,4,5 Safa Shehab,6 Shyam Sreedhara Kurup,1 Mohammed Abdul Muhsen Alyafei,1 Ayesha Salem Al Dhaheri,3 Abdul Jaleel1 1Department of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab Emirates; 2PG and Research Department of Botany, Kandaswami Kandar’s College, Velur, TN, India; 3Department of Nutrition & Health Sciences, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 4Department of Physics, Khalifa University of Science and Technology, Abu Dhabi, United Arab Emirates; 5Department of Physics, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, TN, India; 6Department of Human Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesCorrespondence: Abdul Jaleel, Tel [phone], Email [email protected]: The synthesis of nanoparticles using naturally occurring reagents such as vitamins, sugars, plant extracts, biodegradable polymers and microorganisms as reductants and capping agents could be considered attractive for nanotechnology. These syntheses have led to the fabrication of limited number of inorganic nanoparticles. Among the reagents mentioned above, plant-based materials seem to be the best candidates, and they are suitable for large-scale biosynthesis of nanoparticles.Methods: The aqueous extract of Moringa peregrina leaves was used to synthesize silver nanoparticles. The synthesized nanoparticles were characterized by various spectral studies including FT-IR, SEM, HR-TEM and XRD. In addition, the antioxidant activity of the silver nanoparticles was studied viz. DPPH, ABTS, hydroxyl radical scavenging, superoxide radical scavenging, nitric oxide scavenging potential and reducing power with varied concentrations. The anticancer potential of the nanoparticles was also studied against MCF-7 and Caco-2 cancer cell lines.Results: The results showed that silver nanoparticles displayed strong antioxidant activity compared with gallic acid. Furthermore, the anticancer potential of the nanoparticles against MCF-7 and Caco-2 in comparison with the standard Doxorubicin revealed that the silver nanoparticles produced significant toxic effects against the studied cancer cell lines with the IC50 values of 41.59 (Caco-2) and 26.93 (MCF-7) μg/mL.Conclusion: In conclusion, the biosynthesized nanoparticles using M. peregrina leaf aqueous extract as a reducing agent showed good antioxidant and anticancer potential on human cancer cells and can be used in biological applications.Keywords: plant-based nanoparticles, silver nanoparticles, Moringa peregrina, anticancer, antioxidant activity

Keywords

• plant-based nanoparticles
• silver nanoparticles
• moringa peregrina
• anticancer
• antioxidant activity