Generating ring-shaped engineered heart tissues from ventricular and atrial human pluripotent stem cell-derived cardiomyocytes

Idit Goldfracht; Stephanie Protze; Assad Shiti; Noga Setter; Amit Gruber; Naim Shaheen; Yulia Nartiss; Gordon Keller; Lior Gepstein

doi:10.1038/s41467-019-13868-x

Nature Communications (Jan 2020)

Generating ring-shaped engineered heart tissues from ventricular and atrial human pluripotent stem cell-derived cardiomyocytes

Idit Goldfracht,
Stephanie Protze,
Assad Shiti,
Noga Setter,
Amit Gruber,
Naim Shaheen,
Yulia Nartiss,
Gordon Keller,
Lior Gepstein

Affiliations

Idit Goldfracht: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649
Stephanie Protze: McEwen Stem Cell Institute, University Health Network
Assad Shiti: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649
Noga Setter: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649
Amit Gruber: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649
Naim Shaheen: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649
Yulia Nartiss: McEwen Stem Cell Institute, University Health Network
Gordon Keller: McEwen Stem Cell Institute, University Health Network
Lior Gepstein: Sohnis Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, the Rappaport Faculty of Medicine and Research Institute, Technion‒Israel Institute of Technology, Haifa, Israel, POB 9649

DOI: https://doi.org/10.1038/s41467-019-13868-x
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 15

Abstract

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The cellular composition of previous engineered heart tissue is often heterogeneous. Here, the authors create chamber-specific human pluripotent stem cell-derived cardiomyocytes to form both ventricular and atrial cells before embedding in collagen-based matrix to form ring-shaped engineered heart tissue.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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