E-Cannula reveals anatomical diversity in sharp-wave ripples as a driver for the recruitment of distinct hippocampal assemblies

Xin Liu; Satoshi Terada; Mehrdad Ramezani; Jeong-Hoon Kim; Yichen Lu; Andres Grosmark; Attila Losonczy; Duygu Kuzum

Cell Reports (Oct 2022)

E-Cannula reveals anatomical diversity in sharp-wave ripples as a driver for the recruitment of distinct hippocampal assemblies

Xin Liu,
Satoshi Terada,
Mehrdad Ramezani,
Jeong-Hoon Kim,
Yichen Lu,
Andres Grosmark,
Attila Losonczy,
Duygu Kuzum

Affiliations

Xin Liu: Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA
Satoshi Terada: Department of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA
Mehrdad Ramezani: Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA
Jeong-Hoon Kim: Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA
Yichen Lu: Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA
Andres Grosmark: Department of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA; The Kavli Institute for Brain Science, Columbia University, New York, NY, USA
Attila Losonczy: Department of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA; The Kavli Institute for Brain Science, Columbia University, New York, NY, USA; Corresponding author
Duygu Kuzum: Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, CA, USA; Halıcıoğlu Data Science Institute, University of California, San Diego, La Jolla, CA, USA; Corresponding author

Journal volume & issue: Vol. 41, no. 1
p. 111453

Abstract

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Summary: The hippocampus plays a critical role in spatial navigation and episodic memory. However, research on in vivo hippocampal activity dynamics mostly relies on single modalities, such as electrical recordings or optical imaging, with respectively limited spatial and temporal resolution. Here, we develop the E-Cannula, integrating fully transparent graphene microelectrodes with imaging cannula, which enables simultaneous electrical recording and two-photon calcium imaging from the exact same neural populations across an anatomically extended region of the mouse hippocampal CA1 stably across several days. The large-scale multimodal recordings show that sharp wave ripples (SWRs) exhibit spatiotemporal wave patterns along multiple axes in two-dimensional (2D) space with different spatial extents and temporal propagation modes. Notably, distinct SWR wave patterns are associated with the selective recruitment of orthogonal CA1 cell assemblies. These results demonstrate the utility of the E-Cannula as a versatile neurotechnology with the potential for future integration with other optical components.

CP: Neuroscience

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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