Proteomics Research on Gastric Intraepithelial Neoplasia

Abstract

Objective To investigate the expression of differential proteins in the typical process of gastric intraepithelial neoplasia including normal gastric antrum mucosa, reactive hyperplasia, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and intramucosal carcinoma. Methods We enrolled 150 specimens of different stages of gastric tissues, including normal gastric antrum mucosa, reactive hyperplasia, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and early gastric cancer(each n=30). The expression profiles of total proteins were detected by label-free quantification technology integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Potential biological function of differential proteins was analyzed. Results We screened out eight differential proteins. Compared with normal gastric antrum mucosa, during the process of gastric intraepithelial neoplasia including low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and intramucosal carcinoma, the expression of five proteins LAMN1, ERGIC1, UQCRFS1, UQCRH, CISD1 gradually decreased, and three proteins with increasing expressions were HMGB1, DNA-PKcs, 14-3-3. Conclusion There are many proteins expressed differently in different stages of gastric cancer. They might be expected to be novel early diagnostic and targeted therapeutic biomarkers.

Keywords

• gastric cancer
• dysplasia
• molecular marker
• proteomic