B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors

Daniel F. Kaemena; Masahito Yoshihara; Meryam Beniazza; James Ashmore; Suling Zhao; Mårten Bertenstam; Victor Olariu; Shintaro Katayama; Keisuke Okita; Simon R. Tomlinson; Kosuke Yusa; Keisuke Kaji

doi:10.1038/s41467-023-36097-9

Nature Communications (Jan 2023)

B1 SINE-binding ZFP266 impedes mouse iPSC generation through suppression of chromatin opening mediated by reprogramming factors

Daniel F. Kaemena,
Masahito Yoshihara,
Meryam Beniazza,
James Ashmore,
Suling Zhao,
Mårten Bertenstam,
Victor Olariu,
Shintaro Katayama,
Keisuke Okita,
Simon R. Tomlinson,
Kosuke Yusa,
Keisuke Kaji

Affiliations

Daniel F. Kaemena: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh
Masahito Yoshihara: Department of Biosciences and Nutrition, Karolinska Institutet
Meryam Beniazza: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh
James Ashmore: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh
Suling Zhao: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh
Mårten Bertenstam: Computational Biology and Biological Physics, Lund University
Victor Olariu: Computational Biology and Biological Physics, Lund University
Shintaro Katayama: Department of Biosciences and Nutrition, Karolinska Institutet
Keisuke Okita: Center for iPS Cell Research and Application, Kyoto University
Simon R. Tomlinson: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh
Kosuke Yusa: Stem Cell Genetics, Wellcome Sanger Institute
Keisuke Kaji: Centre for Regenerative Medicine, Institute for Regenaration and Repair, University of Edinburgh

DOI: https://doi.org/10.1038/s41467-023-36097-9
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 16

Abstract

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Induced pluripotent stem cell (iPSC) reprogramming is inherently inefficient. Here the authors identify 24 reprogramming roadblock genes through a CRISPR/Cas9-mediated genome-wide knockout screen including a KRAB-ZFP Zfp266, knockout of which consistently enhances murine iPSC generation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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