Changes in the Microbial Community in Soybean Plots Treated with Biochar and Poultry Litter

Abstract

The application of organic materials that promote beneficial microbial activity is vital to maintaining soil health and crop productivity. We investigated the effect on the soil microbiome of applying biochar (BC), poultry litter (PL), and a combination of biochar and poultry litter (BC/PL) in soybean cultivation at the Red River Research Station (Bossier City, LA, USA). We characterized the microbial profiles, community structure, and co-occurrence network from sequencing data to infer microbial interactions in the soil samples collected in the first and second years of each soil treatment (2016 and 2017, respectively). Our results showed that soil treatments with BC, PL, and a combination of both moderately changed the microbial community composition and structure. In particular, genera significantly affected by the different soil treatments were identified via differential abundance analysis. In addition, canonical correspondence analysis revealed that soil chemical properties, total N in the first year, and total C and pH in the second year influenced the community variability. The differentially enriched bacterial ASVs and co-occurring taxa were linked to nutrient cycling. This study provides insights into the impact of soil carbon amendment on the soil microbiome, a process which favors beneficial bacteria and promotes soybean growth.

Keywords

• soil microbiome
• soybean
• biochar
• soil amendment
• microbial community network