STAR Protocols (Dec 2021)

A macrophage-T cell coculture model for severe tissue injury-induced T cell death

  • Jie Zhu,
  • Jiayu Cao,
  • Arthur Liesz,
  • Stefan Roth

Journal volume & issue
Vol. 2, no. 4
p. 100983

Abstract

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Summary: A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages.For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).

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