Micropropagation of Gynerium sagitatum Aubl. cvs “criolla, “costera” and“martinera”

Abstract

Arrowcane (Gynerium sagitatum Aubl.) (Poaceae) is the fiber source for Colombian most famous handicrafts; however, there are no commercial crops and natural population have decreased jeopardizing artisan activity and associated ecosystems. To provide a mechanism for plant material supply for planting, micropropagation of three arrowcane cultivars was evaluated. Explant from “Criolla”, “Martinera” and “Costera” Cvs were in vitro established and multiplied using different BAP concentrations, followed by in vitro rooting in medium with several NAA levels and transfer to ex vitro conditions. Treatments were distributed with a complete randomized design, data were analyzed with an ANOVA and means separated with Tukey test. BAP statistically increased multiplication rates. NAA promoted adventitious root formation while decreased root length. Complete ex vitro adaptation occurred for all three cultivars indicating the development of an efficient micropropagation protocol to provide large quantity of high quality arrow cane plants.

Keywords

• Brave cane, propagation, In vitro multiplication, in vitro rooting, ex vitro