Desenvolvimento de método analítico por CLAE em comprimidos de Benznidazol para a Doença de Chagas Development of an hplc analytical method for benznidazol tablets for the treatment of chagas disease

Abstract

The analytical method of high performance liquid chromatography (HPLC) for the assay of benznidazole in tablets was developed and validated following the requirements of regulatory agencies. The method used as mobile phase acetonitrile:wather 1:1, a C18 column of 12.5 cm length x 4 mm id, 5 mm particles and lambda=316 nm. The statistical analysis of the results demonstrated that the method satisfies all parameters so as to be considered a safe and efficient analytical alternative of low cost for laboratory routine.

Keywords

• development of method
• validation
• high performance liquid chromatography