A genetic screen for temperature-sensitive morphogenesis-defective <i>Caenorhabditis elegans</i> mutants

Molly C Jud; Josh Lowry; Thalia Padilla; Erin Clifford; Yuqi Yang; Francesca Fennell; Alexander K Miller; Danielle Hamill; Austin M Harvey; Martha Avila-Zavala; Hong Shao; Nhan Nguyen Tran; Zhirong Bao; Bruce Bowerman

doi:10.1093/g3journal/jkab026

G3: Genes, Genomes, Genetics (Mar 2021)

A genetic screen for temperature-sensitive morphogenesis-defective <i>Caenorhabditis elegans</i> mutants

Molly C Jud,
Josh Lowry,
Thalia Padilla,
Erin Clifford,
Yuqi Yang,
Francesca Fennell,
Alexander K Miller,
Danielle Hamill,
Austin M Harvey,
Martha Avila-Zavala,
Hong Shao,
Nhan Nguyen Tran,
Zhirong Bao,
Bruce Bowerman

Affiliations

Molly C Jud: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Josh Lowry: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Thalia Padilla: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Erin Clifford: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Yuqi Yang: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Francesca Fennell: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Alexander K Miller: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Danielle Hamill: Department of Zoology, Ohio Wesleyan University, Delaware, OH, 43015, USA
Austin M Harvey: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Martha Avila-Zavala: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA
Hong Shao: Developmental Biology Program, Sloan Kettering Institute, New York, NY, 10065, USA
Nhan Nguyen Tran: Developmental Biology Program, Sloan Kettering Institute, New York, NY, 10065, USA
Zhirong Bao: Developmental Biology Program, Sloan Kettering Institute, New York, NY, 10065, USA
Bruce Bowerman: Institute of Molecular Biology, University of Oregon, Eugene, OR, 97402, USA

DOI: https://doi.org/10.1093/g3journal/jkab026
Journal volume & issue: Vol. 11, no. 4

Abstract

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AbstractMorphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of ∼200 such Caenorhabditis elegansrib-1rib-2emb-9or1219

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

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